Part:BBa_J119137:Experience

pClone Green (subsequently renamed pClone Red) contains a promoter in reverse orientation as a starting insert between BsaI sites that use CGAC and GCGG sticky ends. The promoter initiates transcription of a GFP gene in reverse orientation preceded by a RBS. Insertion of a functional promoter will lead to transcription of the RFP gene preceded by an RBS. A pClone Green negative control assembly produces colonies that display GFP fluorescence under UV light (left). GGA with a functional promoter yields a mixture of green fluorescent colonies containing the starting pClone Green plasmid and red fluorescent colonies containing the functional promoter (center). GGA with a non-functional or inactive promoter yields a mixture of green fluorescent colonies containing the starting pClone Green plasmid and non-fluorescent colonies containing the non-functional or inactive promoter (right).

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